ELISA试剂盒说明书，前列腺素E2

本试剂仅供研究使用

供应商：上海乔羽生物科技有限公司

目的：本试剂盒用于测定人血清，血浆及相关液体样本中血管紧张素转化酶（ACE）的含量。

ELISA试剂盒说明书，前列腺素E2

实验原理：

本试剂盒应用双抗体夹心法测定标本中人血管紧张素转化酶（ACE）水平。用纯化的人血管紧张素转化酶（ACE）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入血管紧张素转化酶（ACE），再与HRP标记的血管紧张素转化酶（ACE）抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅与样品中血管紧张素血管紧张素转化酶（ACE）过氧呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中人血管血管紧张素转化酶（ACE）浓度。

Determination of angiotensin II in human vascular specimens of the kit using double antibody sandwich (ANG- II) level. Purified human angiotensin angiotensin II (ANG II) antibody package microtiter plate, made of solid phase antibody, to package is microporous monoclonal antibodies are sequentially added in vascular tension angiotensin II (ANG II), and HRP labeled vascular tension angiotensin II (ANG II) antibody binding, formation of antibody, antigen and enzyme labeled antibody complexes, after thorough washing, TMB substrate added color. TMB at HRP enzyme catalyzed conversion into the blue, and in the action of acid into the final yellow. The depth of color samples and angiotensin II (ANG- II) peroxide was positively correlated. Enzyme mark instrument in 450 nm wavelength was used to measure the absorbance (OD), calculated by the standard curve samples of human vascular tension angiotensin II (ANG II) concentration.